Original Article

Cytotoxic Activity of Resveratrol in Different Cell Lines Evaluated by MTT and NRU Assays

  • Hatice Gül ANLAR
  • Merve BACANLI
  • Berkin KUTLUK
  • Arif Ahmet BAŞARAN
  • Nursen BAŞARAN

Received Date: 06.08.2015 Accepted Date: 05.11.2015 Turk J Pharm Sci 2016;13(1):27-34

Oxidative stress is the state of imbalance between the level of antioxidant defence system and production of reac-tive oxygen species (ROS) and is involded in the progression of several diseases such as inflammation, cancer, neu-rodegenerative disorders and cardiovascular diseases. It is suggested that plant polyphenols may act as antioxi-dants and therefore it has anti-cancer activities. Resveratrol (RV), is a naturally occuring polyphenolic compound which is found in many plant species including grapes, nuts, blueberries and raspberries. Data indicated that it has anti-oxidant, anti-inflamatory and anti-cancer activities. But there are also some studies reported that RV has not protective effects aganist cancer. In this study, the cytotoxicity of RV in human breast adenocarcinoma (MDA-MB 231), human cervical cancer (HeLa) and Chinese hamster lung fibroblast (V79) cells were evaluated by Neutral Red uptake assay (NRU) and MTT (3-[4,5-dimethylthiazol-2-yl]-2, -diphenyltetrazolium bromide) assays after incuba-tion at 24 h. We obtained more or the less same results by two cytotoxicity assays. In the concentrations between 2-400 μM, RV seemed not to induce a pronounced cytotoxicity in all cell types. Even at highest concentrations, it showed almost no cytotoxic effects. So the IC50 values were not calculated at the studied concentrations.

Keywords: Resveratrol, Cytotoxicity, Breast cancer, Cervical cancer, MTT assay, NRU assay.