Original Article

Electrochemical Detection of Linagliptin and Its Interaction with DNA

10.4274/tjps.galenos.2021.71354

  • Hüseyin Oğuzhan Kaya
  • Arif Engin Cetin
  • Seda Nur Topkaya

Received Date: 07.01.2021 Accepted Date: 28.02.2021 Turk J Pharm Sci 0;0(0):0-0 [e-Pub]

INTRODUCTION:

Linagliptin is a drug used for the cure of the type 2 diabetes mellitus. In this study, the electrochemical detection of Linagliptin and its interaction with DNA was analyzed for the first time with voltammetric methods by measuring the oxidation currents of adenine bases of DNA before and after the interaction with each other. In addition, the electrochemical properties of the Linagliptin was studied.

METHODS:

The interaction between Linagliptin and DNA were evaluated by using Differential Pulse Voltammetry. Three electrode system which consists of pencil graphite electrode as the working electrode, a reference electrode (Ag/AgCl), and a platinum wire as the auxiliary electrode were used in electrochemical studies. Experimental conditions such as concentration, pH of the supporting electrolyte, and immobilization time were investigated to obtain the maximum analytical signals.

RESULTS:

In our study, the adenine bases of DNA were evaluated as analytical signal obtained at approximately + 1.2 V vs. Ag/AgCl. After Linagliptin-DNA interaction, the oxidation currents of adenine decreased as a proof of interaction. There has been no literature for Linagliptin in connection with DNA. According to our results, diffusion controlled, irreversible redox process consisting of independent oxidation was revealed for Linagliptin. Under optimum conditions, limit of detection was found as 6.7 µg/mL for DNA, and 21.5 µg/mL for Linagliptin, respectively. According to the observations, Linagliptin has a toxic effect on DNA.

DISCUSSION AND CONCLUSION:

We successfully demonstrated that Linagliptin interacts with DNA and its influence on DNA could have a role in the medicinal effect of the drug.

Keywords: DNA, Linagliptin, DNA-Drug Interaction, Electrochemistry, Voltammetry.